Lab Preparation Suite

Interactive calculators for buffer prep, media prep, centrifugation, PCR, serial dilution, gel electrophoresis, autoclaving, freeze-thaw tracking, and wash protocols

Calculators

Buffer Types

Media Types

Cell Protocols

Polymerase Presets

Wash Materials

Buffer Prep

Media Prep

Centrifuge

PCR Master Mix

Serial Dilution

Gel Electrophoresis

Autoclave

Freeze-Thaw

Wash Protocol

🧴 Buffer Preparation Calculator

Configure Recipe

Buffer Type

Target Volume (mL)

Target pH

Concentration

📋 Buffer Reference

Buffer	pKa	pH Range	Default pH
PBS	7.20	6.8–8.0	7.4
Tris-HCl	8.06	7.0–9.0	7.5
TBS	8.06	7.0–9.0	7.6
HEPES	7.48	6.8–8.2	7.4
MES	6.15	5.5–6.7	6.0
MOPS	7.20	6.5–7.9	7.0

Tip: Buffer capacity is optimal within ±1 pH unit of the pKa. The Henderson-Hasselbalch equation is used for pH adjustment calculations.

🧫 Media Preparation Calculator

Configure Media

Base Media

Target Volume (mL)

FBS Percentage (%)

Pen-Strep Percentage (%)

L-Glutamine (mM)

📋 Media Reference

Media	Powder (g/L)	NaHCO₃ (g/L)	Osmolality
DMEM	13.4	3.7	330 mOsm
RPMI 1640	10.4	2.0	290 mOsm
MEM	9.6	2.2	290 mOsm
Ham's F-12	10.6	1.176	300 mOsm
DMEM/F-12	12.0	2.438	310 mOsm
L-15	13.8	0	320 mOsm

Note: L-15 is designed for CO₂-free incubation. All volumes account for supplement displacement.

🌀 Centrifuge Protocol Calculator

RPM ↔ RCF Converter

RPM

Rotor Radius (cm)

RCF (× g)

Cell Type Recommendations

Cell Type

🧬 PCR Master Mix Calculator

Configure Reactions

Number of Reactions

Reaction Volume (µL)

Polymerase

Overage (%)

📋 Polymerase Comparison

Polymerase	Fidelity	Extension	Hot Start
Taq	1×	1 min/kb	No
Phusion	50×	15–30 s/kb	Yes
Q5	280×	20–30 s/kb	Yes
KAPA HiFi	100×	15–30 s/kb	Yes

💧 Serial Dilution Calculator

Configure Dilution Series

Initial Concentration

Unit

Dilution Factor

Number of Steps

Volume per Tube (mL)

⚡ Gel Electrophoresis Analyzer

Gel Recipe Calculator

Gel Type

Gel Percentage (%)

Gel Volume (mL)

Resolution Advisor

Target Size (bp for DNA, kDa for protein)

Molecule Type

♨️ Autoclave Cycle Logger

Log New Cycle

Autoclave ID

Cycle Type

Temperature (°C)

Pressure (psi)

Duration (minutes)

Operator

Items (comma-separated)

📋 Protocol Reference

Cycle	Temp	Pressure	Duration
Gravity	≥121°C	≥15 psi	30–60 min
Pre-Vacuum	≥132°C	≥27 psi	4–18 min
Liquid	≥121°C	≥15 psi	30–60 min
Flash	≥132°C	≥27 psi	3–10 min

Critical: Biological indicators (Geobacillus stearothermophilus) must be included in every load for sterility assurance validation.

❄️ Freeze-Thaw Cycle Tracker

Add Sample

Sample ID